Abstract; The purpose of introducing a combined cycle with gas turbine in power plants is to reduce losses of energy, by effectively using exhaust gases from the gas turbine to produce additional electricity or process. The efficiency of a combined power plant with a gas turbine increases, exceeding 50Percentage, while the efficiency of traditional steam turbine plants is approximately 35Percentage to 40Percentage. Up to the present time, the PID controller has been used to operate this system. However, it is very difficult to achieve an optimal PID gain without any experience, since the gain of the PID controller has to be manually tuned by trial and error procedures. This paper focuses on the neural network tuning of the 2-DOF PID controller with a separated 2-DOF parameter (NN-Tuning 2-DOF PID controller), for optimal control of the Gun-san gas turbine generating plant in Seoul, Korea. In order to attain optimal control, transfer function and operating data from start-up, running, and stop procedures of the Gun-san gas turbine have been acquired, and a designed controller has been applied to this system. The results of the NN-Tuning 2-DOF PID are compared with the PID controller and the conventional 2-DOF PID controller tuned by the Ziegler-Nichols method through experimentation The experimental results of the NN-Tuning 2-DOF PID controller represent a more satisfactory response than those of the previously-mentioned two controllers.