Process Integration onto Biochip Utilizing Novel Materials
Special Symposium - Biotechnology
Invited Session on Biotechnology: Honoring Prof. John Villadsen (S-3)
Keywords: stimuli responsivity, molecular recognision, photoresponsive material, cell manipulation, microchip
Recently, “Proteome” following “Genome” has attracted a great deal of attention in the fields of bioscience and biotechnology. Single molecular analysis has been also feasible in the fields such as the detections of SNPs and specially-functionalized proteins. Taking this situation into account, next we should develop a method and/or device to manipulate and analyze a single cell. To handle a single cell, whose order of magnitude is micro meter, microchip is quite suitable.
We have paid attention to the advantages to use light as a controlling stimulus and are developing novel technologies utilizing photoresponsive materials. At first, I introduce the two-dimensional on-demand cell manipulation technology by light irradiation [1, 2]. Cell selection and precious co-culture of cells are feasible using this technology.
The second topic is about photoresponsive microvalve applied to the remote control of fluids in a microchip. We have already developed the photoresponsive micro valve utilizing a photo- and thermoresponsive hydrogel [3]. Each valve opened within 30 seconds after light irradiation and all valves opened immediately after a temperature rise from 26°C to 46°C.
Finally, we will develop biochips, named “Taylor-made cell array chip”, where cells can be processed, to integrate the above mentioned technologies. We have been already successful to prepare a single channel based on this idea.
Reference
1. Edahiro J.-I., Sumaru K., Tada Y., Ohi K., Takagi T., Kameda M., Shinbo T., Kanamori T., Yoshimi Y.: In-situ Control of Cell Adhesion Using Photoresponsive Culture Surface, Biomacromolecules, 6, 970-974 (2005)
2. Sumaru K., Edahiro J.-I., Ooshima Y., Kanamori T., Shinbo T.: Parallel Manipulation of Living Cells by Using PC-controlled Micro-pattern Projection System, Biosens.Bioelectron., in press
3. Sugiura S., Sumaru K., Ohi K., Hiroki K., Takagi T., Kanamori T.: Photoresponsive Polymer Gel Microvalves Controlled by Local Light Irradiation, submitted to Sens.Actuator A-Phys.
Presented Wednesday 19, 16:30 to 17:00, in session Invited Session on Biotechnology: Honoring Prof. John Villadsen (S-3).
