Organic acid esters such as ethyl lactate belong to a safe, biodegradable and relatively new family of solvents. They can be used as intermediates in chemical reaction and solvents in ink formulations and cleaning applications. An integrated fermentation, extraction and esterification process was developed to produce ethyl lactate ester from ethanol and lactic acid produced during fermentation. Lactic acid was first extracted to the organic solvent containing Alamine 336 and 2-octanol, and then reacted with ethanol to produce ethyl lactate in the extractive solvent using immobilized lipase from Candida antarctica or Yarrowia lipolytica. Ethyl lactate was continuously produced in a plug-flow reactor for 7 days without a decrease in the outlet concentration, suggesting the immobilized lipase was stable. The effects of water content, lactic acid concentration, different alcohols and molar ratio of alcohol to lactic acid on the conversion and productivity of esterification were investigated and will be reported in this paper. The integrated fermentation, extraction and esterification process can economically produce various organic acid esters from biomass.